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文献和实验. Their activities can be measured experimentally with kinase assays that use specific exogenous substrates. The activities of MPF and MAPK are measured using histone H1 kinase and MBP (Myelin Basic Protein) kinase assays, respectively
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium
, such as myelin basic protein. When transplanted into the central nervous system (CNS) of hypomyelinating hosts, oligodendrocyte precursors migrate over considerable distances, and give rise to large numbers of myelinating oligodendrocytes; oligodendroblasts
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