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- 2025年07月12日
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文献和实验Synthesis of Peptides for Use as Immunogens
as an immunogen. Glutathione S-transferase fusion proteins, for example, have been extensively used as immunogens. Alternatively, small synthetic peptides can be made that contain amino acid sequences inferred from that of the gene. Such antipeptide antibodies
，其中包括50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 2 mM MgCl2, 10% glycerol甘油, 1% NP-40, 1 mM NaF, 1 mM EGTA, and 1% protease inhibitor蛋白酶抑制物 cocktail. 裂解液在 15,000g 下离心15 minutes, 上清在 (200μg of protein) 4°C 孵育2 hours ，通常来说伴随着20μg of glutathione S-transferase
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
during apoptosis. The tests were performed on sections of rat, chichen, frog and fish thymus. All the thymus were fixed in Bouin's fixative and embedded in paraffin wax (3). A) Immunocytochemical procedure The low molecular weight DNA fragments
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