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文献和实验next to the protospacer target on the 3′ end. TheCas9 cleavage site is located within the protospacer and positionedat around 3 bp upstream the 5′ end of the PAM, indicating apotential anchor role of the PAM for Cas9-mediated DNA cleavage(Fig. 2a, b ). The PAM sequence
, sharing97% sequence similarity within their kinase catalytic domains(2) . In contrast to most kinases, GSK-3 is inactivated by phosphorylation(2) . GSK-3 is active in nonstimulated cells, whereasother kinases require cell stimulation for activation
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
by sequence and phylogeneticanalysis. 3.1. Primer Design: Manual Method 1. Collect the protein sequences from known members of thetargeted gene family. Perform a BLAST search in the NCBIprotein database using the protein sequence of a member
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