Human Apolipoprotein B (APOB)

载脂蛋白(apolipoprotein B)

人的载脂蛋白有两种表达形式: apoB-48和apoB-100。该基因的序列在肝、肠组织细胞中是相同的,但产物不同。在肝细胞中,产物为4563个氨基酸残基的肽：apoB-100; 但在肠细胞中,产物为只含2152个氨基酸残基的apoB-48, 缺失了apoB-100的N端同LDL受体结合的结构域。原因是在mRNA水平上将2153位的谷氨酰胺的密码子CAA中的C编辑成为U,形成一个终止密码子�UAA,结果使蛋白质的合成提前终止,合成一个新的相对分子质量约为250kDa的蛋白

Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B

subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

with the reagent. As a positive control, use cells set aside at the last step of the reprogramming procedure.        b. Carry out a reverse transcription reaction using 1 µg of RNA (from step 1, above) and the SuperScript® VILO™ cDNA Synthesis Kit following