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- EKU02494
- 2025年07月16日
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
since more values fall into this range. The 6.25 pg/well standard placed in wells A3 through H3, the 3.12 pg/well standard in wells A10 through H10, the 1.56 pg/well standard in wells A4 through H4, and the blank or sample buffer (0 pg/well) in wells A7 through H7
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