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文献和实验Sample Preparation Kit (Pyrosequencing AB) ，将标记有生物素的PCR产物与链亲和素包被的beads（Streptavidin Sepharose™ HP, Amersham Pharmacia Biotech AB, Sweden）相孵育。Gel slurry (4ul) 使用Binding buffer稀释，并与PCR产物在室温下不断摇动混匀10分钟。将beads转移到一块filter plate上，并通过真空过滤（MultiScreen™ Resist
Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)
) is a term used to describe the heritable covalent addition of a methyl group to cytosines at CpG dinucleotides in mammals. While methods for examining DNAm status at specific loci have existed for several years, recent technological advances have begun
System (biotinylated probe): DNA - 1µg dNTPs 10X Mix - 5µl Enzyme 10X Mix - 5µl ddH2 O (from the kit) - to 50µl final Mix well and centrifuge briefly; Incubate 1-2 hours at 16°C. Before stopping the reaction, load 1/10 (5µ
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