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文献和实验Quantitative Profiling of Protein-DNA Binding on Microarrays
on particular motifs. Thus, there is an urgent need for systematic profiling of vertebrate transcription factor binding to DNA. We have developed a high-throughput platform for the quantitative analysis of protein-DNA interactions based on microarray technology.
DNase I Footprint Analysis of Protein‐DNA Binding
I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I?catalyzed hydrolysis
Protein-DNA Binding Affinities by Capillary Electrophoresis
Described in this chapter is a capillary electrophoresis (CE) method for the accurate determination of protein-DNA binding constants (1 ). The advantages of the method include the fast separation and quantitation of protein-DNA complex
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