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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询说明书
- 库存:
10
- 供应商:
上海远慕生物
- 规格:
1 kit (2 x 1 ml reactions)
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文献和实验is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
Buffer TfbI, pH5.8 500ml 1.47g KOAC 4.95g MnCl2 3.73g KCl 0.74g CaCl2
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