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- 2025年12月16日
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- 文献和实验
- 技术资料
- 保存条件：
详询说明书
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
100 mg
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文献和实验Preparation of low density, collagenase-digested splenocytes Protocol
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca2+/Mg2+ (=400U/ml) and 1 ml into 39 ml HBSS/Ca2+/Mg2+ (=100U/ml). Put on ice. 2. Place 5 ml of the 100U/ml solution in a 10 cm dish. Over a dry dish, inject spleens
Preparation of Low Density, Collagenase-Digested Splenocytes (Hedrick Lab, UCSD Cancer Center)
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ (=100U/ml). Put on ice. 2. Place 5 ml of the 100U/ml solution in a 10 cm dish. Over a dry dish, inject spleens
Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ (=100U/ml). Put on ice
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