- 询价
- Roche
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- 4719948001
- 2025年12月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询说明书
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
1 kit (100 ml lysis reagent and 20 Complete Mini, EDTA-free Protease Inhibitor Cocktail Tablets)
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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
by centrifugation. Transfer appropriate volume of culture to a 15 ml centrifuge tube and centrifuge at 5,000 x g for 10 minutes. Alkaline-SDS Lysis of Bacterial Cells: 2. Decant or aspirate medium. To ensure that all traces of the medium are removed, use
As mentioned in Chapter 9 , Freund’s complete adjuvant (FCA), produced by the Statens Seruminstitut (Copenhagen, Denmark), was chosen as the standard adjuvant preparation because of its extensive use in experimental vaccines in animals
of plasmids, we have used everything from CsCl-banded DNA , quick prep DNA , and heat denatured, transformed bacteria. In the latter case, a single bacterial colony (or as small a portion of a frozen stock as possible) was added to 0.5 ml of TE, vortexed
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