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- 文献和实验
- 技术资料
- 保存条件:
详询说明书
- 库存:
10
- 供应商:
上海远慕生物
- 规格:
25 ml
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文献和实验Purification of Plasmid from 50 ml-culture
4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin
Celestine Blue 2.5g Ferric Ammonium Sulphate (Iron Alum) 25g Glycerin 70ml Distilled water 500ml Dissolve ferric ammonium sulphate in cold distilled water. Add celestine blue and boil for a few mins. Cool, filter and add glycerin
A protocol for cleaning and reusing the large 25 x 25 cm plates
). For our sink, I fill the sink about 3/4 full (approximately 50 litters) and add 500 to 1000 ml Clorox. (500 ml gives a final concentration of about 1% bleach). I let the plates soak in the dilute bleach solution for approximately 1/2 hour
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