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呋喃它酮快速检测卡

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  • 国内
  • SU-F00021
  • 2025年11月26日
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    上海瓦兰生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存：

      大量

    • 供应商：

      上海瓦兰生物

    • 检测范围：

      不限

    • 检测方法：

      酶联免疫法

    • 应用：

      科研单位

    • 适应物种：

      不限

    • 标记物：

      呋喃它酮

    • 样本：

      液体

    • 规格：

      50t

    呋喃它酮快速检测卡

    Sample collection and storages
    Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
    Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
    Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
    Note: The samples should be centrifugated adequately and no hemolysis or granule was allowed.
    Materials required but not supplied
    1. Standard microplate reader(450nm)
    2. Precision pipettes and Disposable pipette tips.
    3. 37 ℃ incubator
    Precautions
    1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
    2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
    3. Mix all reagents before using.
    Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
    Materials supplied
    Name 96 determinations 48 determinations
    Microelisa stripplate 12*8strips 12*4strips
    Standard 0.3ml 0.3ml
    Sample diluent 6.0ml 3.0ml
    HRP-Conjugate reagent 10.0ml 5.0ml
    20X Wash solution 25ml 15ml
    Chromogen Solution A 6.0ml 3.0ml
    Chromogen Solution B 6.0ml 3.0ml
    Stop Solution 6.0ml 3.0ml
    Closure plate membrane 2 2
    User manual 1 1
    Sealed bags 1 1
    Note: Standard concentration was followed by:
    8、4、2、1、0.5、0 ng/ml.
    Reagent preparation
    20×wash solution:Dilute with Distilled or deionized water 1:20.
    Assay procedure
    1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
    2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
    3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.
    4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
    5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
    7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not


    appear uniform, gently tap the plate to ensure thorough mixing.
    8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
    Calculation of results
    1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
    2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
    3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
    4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
    5. The sensitivity by this assay is 0.1 ng/ml.
    6. Standard curve


    Storage： 2-8℃.
    validity： six months.

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

    兽药残留系列
    货号 产品名称 规格 价格
    SU-F00010 呋喃唑酮快速检测试剂盒 96T/盒 3200
    SU-F00011 呋喃西林快速检测试剂盒 96T/盒 3200
    SU-F00012 呋喃妥因快速检测试剂盒 96T/盒 3200
    SU-F00013 呋喃它酮快速检测试剂盒 96T/盒 3200
    SU-F00014 喹乙醇检测试剂盒 96T/盒 3200
    SU-F00015 己烯雌酚快速检测试剂盒 96T/盒 3200
    SU-F00016 地塞米松快速检测试剂盒 96T/盒 3200
    SU-F00017 三聚qing胺快速检测试剂盒 96T/盒 3200
    SU-F00018 呋喃西林快速检测卡 50T/盒 1800
    SU-F00019 呋喃妥因快速检测卡 50T/盒 1800
    SU-F00020 呋喃唑酮快速检测卡 50T/盒 1800
    SU-F00021 呋喃它酮快速检测卡 50T/盒 1800
    Sample collection and storages
    Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
    Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
    Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
    Note: The samples should be centrifugated adequately and no hemolysis or granule was allowed.
    Materials required but not supplied
    1. Standard microplate reader(450nm)
    2. Precision pipettes and Disposable pipette tips.
    3. 37 ℃ incubator
    Precautions
    1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
    2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
    3. Mix all reagents before using.
    Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
    Materials supplied
    Name 96 determinations 48 determinations
    Microelisa stripplate 12*8strips 12*4strips
    Standard 0.3ml 0.3ml
    Sample diluent 6.0ml 3.0ml
    HRP-Conjugate reagent 10.0ml 5.0ml
    20X Wash solution 25ml 15ml
    Chromogen Solution A 6.0ml 3.0ml
    Chromogen Solution B 6.0ml 3.0ml
    Stop Solution 6.0ml 3.0ml
    Closure plate membrane 2 2
    User manual 1 1
    Sealed bags 1 1
    Note: Standard concentration was followed by:
    8、4、2、1、0.5、0 ng/ml.
    Reagent preparation
    20×wash solution:Dilute with Distilled or deionized water 1:20.
    Assay procedure
    1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
    2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
    3. Add Sample: Add testing sample 10μl Then add sample diluent 40μl to testing sample well; Blank well doesn’t add anyting.
    4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.
    5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
    7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not


    appear uniform, gently tap the plate to ensure thorough mixing.
    8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
    Calculation of results
    1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
    2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
    3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
    4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
    5. The sensitivity by this assay is 0.1 ng/ml.
    6. Standard curve


    Storage： 2-8℃.
    validity： six months.

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

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