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Cellular Mechanisms: Differentiation: BMP2, CADM1 (TSLC1), CCND2, CHST11, DHH, DKK1, EFNB1, EPHA4, EPHB3, FOXA2 (HNF3B), GATA2, GATA6, HAND1, HES1, IL6R, IRX3, JUN, KLF4, OTX2, PDGFRA, PMP22, RARB, SALL1, SOX17, THPO, TRIM10, TRIM15, UCP1, WNT5A.
Signal Transduction: BMP2, CDC42EP1, CDKN1A (p21CIP1/WAF1), CHST11, CXCL16, CXCR7, DKK1, DKK2, EPHA4, EPHB3, GAS6, HES1, HEY1, IGFBP1, IL6R, IRS4, JUN, OTX2, PDGFRA, PRKCH, PRKG2, PTPRA, RARB, RASD1, RASSF1, SMOC2, SOX17, TACSTD2, TCF7, TNFRSF19, WNT5A.
Cell Cycle: BMP2, CCND2, CDKN1A (p21CIP1/WAF1), DHH, ESX1, G0S2, JUN, RASSF1.
Apoptosis: CADM1 (TSLC1), CDKN1A (p21CIP1/WAF1), CHST11, GATA6, IL6R, JUN, RARB, RYBP, SERPINB2 (PAI-2), TNFRSF19.
Extracellular Matrix Organization: CTSA, LOX, PDGFRA, TIMP3.
Chromatin Modification: BMI1 (PCGF4), CBX8, HMGN2, RBBP7.
Other: CDH11, CYP1B1, DGAT2, FAM65A, HS3ST1, HS6ST2, MGLL, MYO5B, NID2, NPTX1, THBD, TMEM66, USP3, VSNL1.
Cellular Functions: Transcription Factors: ATF3, ESX1, FOXA2 (HNF3B), GATA2, GATA3, GATA4, GATA6, HAND1, HEY1, HOXA10, HOXA5, IRX3, JUN, KLF4, MEIS2, OTX2, RARB, SALL1, SATB1, TRIM15, TSHZ1.
Transporters: KCNA1, SLC30A4, SLC34A2, TRPM3, UCP1.
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文献和实验and sequencing For PCR primer pair selection, you can choose a target range of the template sequence to be amplified In selecting appropriate primers, PRIME allows you to specify a variety of constraints on the primer and amplified product sequences. -upper
全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fine-Tiling Array CGH)
of breakpoints to an interval that can be validated by PCR amplification and sequencing. The selected regions need not be contiguous or even within the same chromosome. For example, the copy number changes identified in a whole-genome array CGH experiment
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
designed to amplify a target between 150 and 600 base pairs. Targets smaller than 200 bp are difficult to resolve on agarose gels, and larger targets place a greater burden on the investigator to optimize PCR conditions. The critical aspect for RT-PCR
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