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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dihydrochloride Kodak #X8748 DNA PAGE % gel 3.5 5.0 8.0 12.0 20.0 30% acryl stock 11.6 16.6 26.6 40.0 66
High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
Fast and reliable mini-prep RNA extraction from Neurospora crassa
products. Lane 1: 14C-labelled marker proteins, molecular wieghts are indicated on the left (Mr x 10(-3). Lanes 2 and 3: RNA prepared from mycelia grown in rich or ammonium depleted medium respectively. RNA probes correspond to lanes 1 and 2 in Fig
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