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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dihydrochloride Kodak #X8748 DNA PAGE % gel 3.5 5.0 8.0 12.0 20.0 30% acryl stock 11.6 16.6 26.6 40.0 66
High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
After thawing, 3mls of whole blood are transferred to a sterile conical centrifuge tube (15ml volume) to which 9mls of 1 x erythrocyte lysing buffer (0.155M NH4 Cl; 10mM KHCO3 ; 0.1mM Na2 EDTA; pH 7.4) must be added. The solution is left for 10
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