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文献和实验) containing 4mM L-glutamate, 4.5 mg/ml glucose and 0.11 mg/ml sodium pyruvate (Invitrogen UK, Cat no: 41966-029), supplemented with 100 U/ml Penicilin and 100 µg/ml Streptomycin (1% v/v Penicilin/Streptomycin solution, Invitrogen UK, Cat no: 15140-122
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, gelatin or BSA to 100ug/ml, and/or non-ionic detergents such as Tween-20 or Nonidet P-40 or Triton X-100 (0.05 - 0.10% v/v) (Innis and Gelfand, 1990)。 Modern formulations may differ considerably, however - they are also generally proprietary. PCR
) (10 ). Amino acid alignment of full-length GHRs reveals a relative high degree of identity (35�40%) among tetrapods and non-salmonid fish GHRs (GHR type I). Several authors have postulated a divergent evolution of salmonid GHRs (GHR type II
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