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上海希言科学仪器有限公司
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
by inversion. 8、Spin in a microfuge for 10 minutes.A small white/clear pellet will form. 9、Remove supernatant.Wash with 70% ethanol. 10、Dry pellet and resuspend in 50µl water containing 20 µg/ml RNase A. STET 8% sucrose 5% Triton X-100
I buffer (Tris pH8.4, 20 mM MgCl2 , 500 mM KCl) 5 µL DNAse I (RNAse free) q.s. 100 µL with DEPC H2 O Incubate at room temp. x10 min. Immediately proceed to next step. RNeasy Mini Prep Procedure
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