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Nickel Plated Brass, 1/4"NPT Hydrogen
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文献和实验double-stranded plasmids are picked with sterile toothpicks into media and incubated at 37degC for 24 hours with shaking at 350 rpm. These cells are harvested by centrifugation and the pellets are either manually or robotically resuspended by the addition
double-stranded plasmids are picked with sterile toothpicks into media and incubated at 37degC for 24 hours with shaking at 350 rpm. These cells are harvested by centrifugation and the pellets are either manually or robotically resuspended by the addition
Reverse Ligation Mediated RT -
d/dd NTP mixes (20µM dATP, dCTP, dTTP, 7-deaza dGTP: A mix 350µM ddATP, C mix 200µM ddCTP, G mix 30µM ddGTP, T mix 600µM ddTTP). Ethanol, ice cold. Light mineral oil. Transcription and purification of RNA linker
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