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文献和实验Purification of Ncd Motor Domain Protein
another aliquot of PMSF and protease inhibitors halfway through the incubation. 5. Centrifuge at 18,000 rpm (39,000 x g) and 4°C for 20 min in the Sorvall SS-34 rotor. 6. Transfer clear yellow supernatant
Purification of KHC Motor Domain Protein
) and 4°C for 20 min in the Sorvall SS-34 rotor. 6. Transfer clear yellow supernatant to Beckman TLA ultracentrifuge tubes. Centrifuge at 80,000 rpm (270,000 x g) and 2°C for 30 min in the TLA 100.3 rotor in a Beckman TLX
Purification of Ncd Motor Domain Protein
another aliquot of PMSF and protease inhibitors halfway through the incubation. 5. Centrifuge at 18,000 rpm (39,000 x g) and 4°C for 20 min in the Sorvall SS-34 rotor. 6. Transfer clear yellow supernatant
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