Shodex DS-613 Column, 6 µm, 6

Column Buffers: CREB

of buffers prior to use. Storage Buffer for Column For 500ml 10mM Tris ph 7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column - Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol

Chromatin IP (CHIP assay)

PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction:      1 x 95 °C, 2 min      21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min      1 x 72

Linear amplification of limiting amounts of RNA for gene expression studies

with DEPC-H2 O, in case of a bigger volume, speedvac to 8µl. In vitro transcription (IVT) 1 (in RNAse-free PCR tubes) Add the IVT mix consisting of 4µl 5x IVT reaction buffer, 6µl 25mM rNTP mix and 2µl T7 enzyme mix to the ds cDNA (room