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文献和实验Mag-Bind RX Plasmid Isolation Protocol
to each well. Wash the Mag-Bind particles by pipetting up and down for 10 times . Place the plate on the magnetic separation stand to magnetize the Mag-Binds particles. Aspirate the supernatant. 13. Optional: Remove the plate from magnetic separation stand
Measurement of Cell Adhesion Under Static Conditions
forces generated by washing the wells. This protocol introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity thereby eliminating uncontrolled shear forces and passage of adherent cells through a liquid/air interface
Measurement of Cell Adhesion Under Static Conditio
through a liquid/air interface. The cells are loaded with a fluorescent dye (6-carboxyfluorescein diacetate) for detection although other methods such as radioactive labels may be used. This protocol is also useful for assaying molecules that promote or inhibit
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