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上海希言科学仪器有限公司
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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008)Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
if you have any questions or comments Set Up 1. Drain any water left in the fermentor. Open filling port cap on headplate. Remove drain port steam cap, open drain port and collect liquid. Close drain port and replace steam cap.
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