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文献和实验Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of Chromatin LEVEL II Materials Bovine or porcine brain 0.25 M Sucrose containing 0.0033 M calcium
A novel method of growing fungi for DNA extraction
Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks, Roux bottles or even microfuge tubes (Cenis 1992 Nucl. Acids Res. 20:2380). Growing fungal cultures in liquid may require
Fast and reliable mini-prep RNA extraction from Neurospora crassa
were stamped out using an inverted Pasteur pipette and frozen. The procedure of extraction given below is described for Eppendorf tubes but it is also possible to scale up the volumes. All manipulations were performed at room temperature if not stated
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