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文献和实验Agrobacterium growth and transformation (农杆菌培养及转化)
). Method 1: Freeze/thaw shock transformation. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy. 1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap
Agrobacterium growth and transformation
transformation. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy. 1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap tube (Falcon tube). Grow @ 30°C overnight
Principles of Aseptic Technique
a. The animal's respiratory fu nction should not be compromised by overextension of forelegs stretched towards the head, or by excessive body tilt which causes pressure from the abdominal organs on the diaphragm. b. Limbs should not be extended
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