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文献和实验Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin
4 and 5 two more times for a total of 4 washes Add 10 ml 2x NETS and store at 4°C Prep cellulose the day before you need it mRNA prep Dilute 1 mg total RNA to 1 ml with 10mM Tris pH7.4 in c/f tube In a 2 ml BioRad mini column(#731-1550
偶然发现的一个pQE30系统表达蛋白纯化案例,帖出来和大家分享。 Purification of LF. 诱导与表达 E. coli SG13009(pREP4) carrying the recombinant plasmid pPG-LF1 was grown at 37°C in Luria broth with 100 μg of ampicillin and 25 μg of kanamycin per ml at 250 rpm. When the A600
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