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文献和实验Transformation of E. coli by Electroporation
ionic strength and a high resistance. The DNA may be purified by either precipitation or dialysis. Purifying DNA by Precipitation: 1. Add 5 to 10 μg of tRNA to a 20 μl ligation reaction. Add 22 μl 5M ammonium acetate. Mix well. 2. Add 100 μl
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
and to use PCR to determine that the cells contain your construct. PCR is necessary here because the Ti plasmid is single copy and you can barely see it on agarose gels. 1. Grow cells overnight in 5 ml LB or YEP with antibiotics. For pMONs in ABI - 50
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