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上海希言科学仪器有限公司
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文献和实验the lyophilized protein from step 3 in 10 M urea-0.9 N acetic acid-0.5 M -mercaptoethanol (to a final concentration of 500 micrograms protein per 100 µl of buffer) and incubate at room temperature for 12-14 hours prior to the next step. Apply
mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
the following DNA dilution, and aliquot 35 μl into ten 1.5 ml microcentrifuge tubes: DNA 100 μg 10X TM buffer 35 μlsterile ddH2 O q.s. Final Volume 350 μl 2. To determine the optimal sonication conditions, sonicate the DNA samples in five of the tubes
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