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文献和实验CCMB buffer to make chemical competent cell
and kanamycin appear to do fine with 1 hour growth Plate 20 μl on AMP plates using 3.5 mm glass beads Transformation efficiency is 15 x colony count x 105 There is anecdotal evidence that refreezing partially used cell aliquots dramatically reduces
【共享】流式 Overview of Flow Cytometry
distinct subpopulations of cells on the basis of these measured characteristics, usually by electrostatic deflection. This separation technology is called electronic cell sorting.Flow cytometry has two general applications—quantitative analysis and cell
Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis).
Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) and Radetzky (1990). Cell cultures at four days post-subculture are ground at 4 degrees Celsius in a mortar and pestle with sand
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