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文献和实验. SPRi experiment The spotted biochip was inserted into the SPRi-PlexII™ instrument from HORIBA Scientific. The system is equ-ipped with a 200 μL sample loop,a continuous flow pump and an in-line degasser. The flow rate
. After incubation, 100μl of each reaction were transferred into a sample tube and diluted 1:10 in appropriate phosphate buffer to reduce the concentration of NaCl to 150mM. Diluted reactions were anaylzed for HA activity.Batch AdsorptionBatch adsorption
including the point of sample introduction. In an on-column injection, the needle must enter the column inside the oven. The injector used has a butterfly valve, which is opened as the turret of the autosampler rotates over the injector, . The sample is
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