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文献和实验High Efficency Yeast Plasmid Rescue
Grow yeast overnight. Spin down 1.5 ml of culture in microfuge tubes. Decant. Add 250 µl of Qiagen buffer P1 and about 250 µl of glass beads to each tube. Beat or vortex on high for 3-5
Miniprep/Kit-free high-throughput protocol
to pellet the cells. Remove and discard the supernatent. Add 300 μL STET buffer, and resuspend cells by vortexing. Add 10 μL lysozyme (10 mg/mL), vortex, and submerse in boiling water for 40 sec. Spin for 30 min in a tabletop
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
20-25 times. Incubate the mixture at 37°C for approximately 10 minutes. 6. Cool the sample to room temperature. Add 1/3 Volume of Buffer PCP to the cell lysate. Vortex vigorously at high speed for 30 seconds to mix. Some protein clumps
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