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文献和实验RADIOLABELING OF PROBES FOR ELECTROPHORETIC MOBILITY SHIFT ASSAYS
,flick gently to mix Place the tube on a rack in the 37℃ incubator for 1h. IN THE HOOD,behind a shield,add 60μl of STE to the reaction. Equilibrate a push column by adding 75μl of STE and forcing this through with a 10cc disposable syringe until a drop
Isolation of murine splenocytes
listed are for use with one mouse. Supplies 15ml conical tube 60mm petri dish 5ml pipet 100μm cell strainer (can substitute autoclaved fine nylon mesh for Protocol B) 3mL sterile disposable syringe, no needle
Radiolabeling of probes for electrophoretic mobility shift assays
to the reaction. 9、Equilibrate a push column by adding 75 ul of STE and forcing this through with a 10 cc disposable syringe until a drop comes out of the bottom of the column . Mount the column on the beta shield device, and place a clean eppendorf tube
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