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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 High-Energy Nd:YAG Laser Mirror, 25.4 mm Diameter, 45°, 532 nm Newport 10Q20HE.2 4 SUPREMA Optical Mount, 1.0 inch diameter
-mm Nalgene filter. Divide into aliquots and store at -20ºC. Pick a single bacterial colony (2-3 mm in diameter) from a plate that has been incubated for 16-20 hours at 37ºC. Transfer the colony into 25 ml of LB
Lipoprotein Isolation - First week
50 rotor in the opposite positions and place the rotor in the ultracentrifuge. 16. Make sure the lid is closed. Turn on the vacuum of the centrifuge. Set the run conditions to 10 C, brake on, speed 45,000, time 4.5 h. 17. Spin at 45,000
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