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文献和实验A. Plating: To sterilize glass coverslips, dip in ethanol and flame. We use 22x22x1 mm3 coverslips and put them in 6-well plates. Seed 100,000 cells per well overnight and fix the next day. B. Fixation: Remove the media
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Live imaging with Drosophila tissue culture cells
with most Drosophila tissue culture cells as long as they are semi-adherent. I tested a variety of different Drosophila Schneider S2 cells for their growth behavior and found that most of them had some difficulties undergoing and completing cytokinesis, producing
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