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文献和实验Sample preparation (preparative gels)
of 3000 µl of human CSF was mixed with 6000 µl of ice cold acetone and centrifuged at 10000 g at 4o C for 10 minutes. The pellet was mixed with 500 µl of a solution containing urea (8 M), CHAPS (4% w/v), DTE (65 mM), Resolytes 4-8 (2 % v/v) and a trace
Cyanine Dye Purification Protocol
may be loaded up to 150 A260 units of sample (based on the A260 reading for both labeled and unlabeled dNTPs in the filtrate), lyophilized down to 3-5 mL (volumes of 6.5-7.5 provided reasonable yields, and volumes of u p to 10 mL have been successful
: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
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