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文献和实验Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
(6) food wrap Reagents: solid urea 67.5 g 40 % acrylamide (19:1) 20.25 mL 5 x TBE 27 mL
Screening lambda cDNA or genomic libraries
need 6.6 ml top agarose (use a bigger tube and a 50-55° water bath).to titer (on 100 mm plates):Put various dilutions of phage in 100 µl 1X phage buffer.Always do a no phage control! This will help you distinguish between dilutions that give you no phage (look
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