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文献和实验setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
In Situ Hybridization Using Digoxigenin Labeled Probes
and Washes: 1) Denature the hybridization probe by boiling for 3 minutes and chill on ice 2) Apply 5 µl of hybridization solution on the slides and distribute the solution over the chromosomes with a 18 x 18 mm coverslip. Try to avoid trapping air
三句话读懂一篇 CNS:女性易患自身免疫性疾病的机制、雄激素高的男性更小气...
., Huang, X., Liu, Z. et al. Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas. Nat Metab 3, 352–365 (2021). https://doi.org/10.1038/s42255-021-00364-0.4. Jianxin Ou et al. Testosterone reduces generosity
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