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文献和实验Preabsorbing Antisera with C. elegans Acetone Powder
, add 3 volumes of 0.1 M NaCl. Lyse the worms by sonication. I've been using the microtip of a Branson sonicator set at the microtip limit. I use 8 minutes of sonication for a 20 ml suspension, with the machine on a 50% 2 second cycle. Examine the sample
Vortex 5 min Microfuge 15 min, 14,000rpm (12,300 rpm on larger-radius centrifuge) 4. Label second set of eppendorfs Add 1 ml of 100% EtOH 5. Transfer 500 l of supernatant to these tubes Invert several times
Vortex 5 min Microfuge 15 min, 14,000rpm (12,300 rpm on larger-radius centrifuge) 4. Label second set of eppendorfs Add 1 ml of 100% EtOH 5. Transfer 500 l of supernatant to these tubes Invert several times
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