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文献和实验Q R S T U V W X Y Z Chloroplast Mitochondrion Others (intials are not capital) CUTG: Codon Usage Tabulated from GenBank (ftp distribution
to each 40 µL digestion rxn: (recipe is for one ligation rxn) 1.0 µL EcoRI-adapter 1.0 µL MseI-adapter 1.0 µL 10 mM ATP 4.0 µL 5x-OPA+-BSA 1 U T4 ligase (Pharmacia) Q.S. to 10 µL w/ dH20 - Incubate @ 37° C for 3 hrs to O
AFLP: not only for fingerprinting, but for positional cloning
-adapter 1.0 µL MseI-adapter 1.0 µL 10 mM ATP 4.0 µL 5x-OPA+-BSA 1 U T4 ligase (Pharmacia) Q.S. to 10 µL w/ dH20 - Incubate @ 37° C for 3 hrs to O/N 2.0 Pre-amplification of template DNA [Note 1: This pre-amplification step helps to "clean
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