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文献和实验RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus
immunodeficiency syndrom (AIDS) patients (e.g., CMV-associated retinitis and pneumonia), and possibly cardiovascular diseases such as atherosclerosis. This chapter describes the utilization of RNase P ribozyme—specifically, M1GS ribozyme, as a gene-targeting agent
can be made competent for transformation by treatment with ethylene glycol and dimethyl sulfoxide (DMSO) and then frozen in small aliquots and stored at - 70o C. While the highest transformation efficiencies are obtained with freshly grown cultures, the moderately
by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium
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