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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
, set the pulse frequency to 100, continuous mode, and reduce power to a minimum using the half-wave plate. Use a Post-It note or lens paper to visualize the laser beam. 5) Adjust and fix the kinematically mounted mirrors in sequence
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
of four square wave pulses. Bubbles should be visible on the cathode. An amperage value of at least 0.01 A should be displayed after the completion of the pulse series. iii. Decrease the voltage setting to 4 V after conductance
-quadrupole mass spectrometers are most commonly used to obtain amino acid sequences. In the first stage of analysis, the machine is operated in MS scan mode and all ions above a certain m /z ratio are transmitted to the third quadrupole for mass analysis (Fig
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