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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
. Thus, a sixteen base sequence will statistically be present only once in every 416 bases (=4 294 967 296, or 4 billion): this is about the size of the human or maize genome, and 1000x greater than the genome size of E. coli. Thus, the association of a greater
Metabolic labeling Immunoprecipitation
survey meter.Wipe test + LSC is necessary. Chemical properties... Use either 35S-methionine or cysteine or both.Recommend using Expre 35 S35 S Protein labeling mix (NEN).Before opening the cap of the vial,thaw the solution completely and open the cap
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