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文献和实验. If you could get by with less, Jonathan Jones would have done so!Stocks0.25N HCl0.25N NaOHTris buffer:0.5M Tris pH 8.00.25% Nonidet P-40LEAF PCR ON ARABIDOPSIS TISSUE WITHOUT ALKALINE TREATMENTPreparation of Master mix:1 x Taq-buffer1.5 mM MgCl2200 mM of each dNTP1 mM each primer0.5 ml
ES / MEF cell culture and electroporation of targeting construct
contact. Having the safety stand connected to the rear of the unit using the cables supplied, turn on the power switch. Set electroporator (BTX 600 or equivalent) as follows: 00V/Capacitance and resistance, 500uF capacitance timing, 360 ohms R8
Preabsorbing Antisera with C. elegans Acetone Powder
, add 3 volumes of 0.1 M NaCl. Lyse the worms by sonication. I've been using the microtip of a Branson sonicator set at the microtip limit. I use 8 minutes of sonication for a 20 ml suspension, with the machine on a 50% 2 second cycle. Examine the sample
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