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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
Bacterial Expression of GST-fusion Proteins
well with shaking at 37o C until OD at 600nm is ~0.6 (usually about 4 hrs). 3. Add the appropriate amount of IPTG stock solution to the culture to obtain a final IPTG concentration of ~2mM. (Add 4mls water to an unopened bottle= 1M. Induce 1L culture
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
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