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文献和实验Agrobacterium growth and transformation (农杆菌培养及转化)
Corex tubes). 4. Discard supernatant, drain inverted for 30-60 seconds, and resuspend pellet in 1 ml of ice cold 20 mM CaCl2. Dispense 0.1 ml of bacterial suspension into each of two pre-chilled 1.5 ml. microfuge tubes on ice. One is a control.
Agrobacterium growth and transformation
, and resuspend pellet in 1 ml of ice cold 20 mM CaCl2. Dispense 0.1 ml of bacterial suspension into each of two pre-chilled 1.5 ml. microfuge tubes on ice. One is a control. 5. Add 1 ug of plasmid DNA to one tube and nothing to the other the control) and mix
to the injection procedure and being on the maze in order to control for these effects. Note: always wear rubber gloves & lab coat. 1. Prepare saline injection ~ (.2 ml.). 2. Give the rat an interperitineal injection. 3. Repeat process
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