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文献和实验preservatives (see Hint #2 ). B. Use of the Dialysis Tubing 1. After washing the tubing (see Protocol ID#113 ), calculate the length of tubing necessary to contain the volume of your protein sample and cut the tubing to length, leaving an extra inch
Chick Chorioallantoic Membrane (CAM) Assay
for gross evaluation. For injection, borosilicate glass capillaries (OD 1.0 mm, ID 0.75 mm; Sutter Instrument Company, Novato, CA) were prepared with P-87 micropipette puller (Sutter Instrument Company). Needles were connected with Tygon tubing (ID 1/32
Myosin Light Chain Preparation
the 8 liter and the 28 liter beakers. 5.Put ETOH in the cold room. 6.Refrigerate or equilibrate the DE52 to be used. 7.Find parts to and assemble the 5½ cm ID column. 8.Make sure there is plenty of cold water in the cold room. 9.Thaw 8 M urea. Solutions
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