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文献和实验Isolation of micro-RNA (miRNA)
the frozen tissue sample in solution D using a Dounce, micro-Dounce, or a mechanical microhomogenizer. 5. Transfer the homogenate to a microcentrifuge tube. Note Hereafter, the volume of homogenate used is defined as "volume D" (e.g., in the sample
AFLP: not only for fingerprinting, but for positional cloning
acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs
AFLP: not only for fingerprinting, but for positional cloning
AFLP results if one follows the protocols below.] 1.2 Restriction of DNA (Recipe is for one sample) 0.5 µg genomic DNA (total nucleic acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer
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