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文献和实验.16 112.05 Dynabeads M-280 Streptavidin Supplied at 6.7x108 beads per ml (10mg/ml) in PBS, pH7.4 with 0.1% BSA and 0.02% sodium azide. Isolation of any biotinylated target. Applications include DNA, RNA and protein isolation
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Viral RNA extraction-Molecular Biology
and virus.The mice tissues were fix in formalin for two weeks, paraffin embed allowfullscreen='true'ed. I used 5um x 50 sections, so i will give me an average of 20 to 25mg tissue.I used the above kit to extract RNA and I manage to get 46.5ug/ml and A260
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