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文献和实验Aggregation of ES Cells and Eight Cell Stage Embryos
and 3 cc syringe (filled) to run a small amount of silicone (Sigmacote) through the inside of a piece of capillary glass, allowing excess Sigmacote to drip into a 10ml tube. This is repeated with each piece of glass and the glass placed in a measuring
In Situ HYBRIDIZATION TO TISSUE SECTIONS
2O. Pronase treat: -Remove the slides from the rack and place horizontally in a tray. -With a Pasteur pipet, carefully drip ‰0.25mg/ml pronase solution onto the sections and incubate 10' (thhe incubation time starts with treatment
Principles of Aseptic Technique
ll be the recommendations for Aseptic Surgery contained in the Guide for the Care and Use of Laboratory Animals . The Guide states: "Functional areas for aseptic surgery should include a separate support area, a preparation area, the operating
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