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文献和实验Purifying Protein from Inclusion Bodies
for each gram of cells and fully resuspend the cell pellet. 12.Add the same amount of 100 mM PMSF as you did in step 6 and 10μl Triton X-100 per ml of solution and resuspend the pellet. 13.Spin at the highest speed possible (~20,000 rpm)for 20 min
μl are fine. 6. The exchange of solution components between the protein sample and the Dialysis Buffer is most efficient if carried out for longer than 1.5 hr. This should be empirically determined. However, an overnight incubation
Chick Chorioallantoic Membrane (CAM) Assay
Dickinson, Bedford, MA) was diluted to a final concentration of 10 mg/ml with DMEM and directly pipetted onto meshes. Nylon mesh with 250 μm2 openings were cut into 4 mm x 4 mm squares and autoclaved. In preparation for polymerization, meshes were placed
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