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文献和实验Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b
of a buffer such as phosphate buffered saline (PBS) or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results. Again for two rabbits, prepare RIBI (or Freund's) adjuvant by warming
Purifying Protein from Inclusion Bodies
for each gram of cells and fully resuspend the cell pellet. 5.Transfer the suspended pellet to 50 ml conical centrifuge tubes. 6.Add enough 100 mM PMSF to make a 1 mM final solution (10μl 100 mM PMSF/ml of solution). 7.Add 16μl of 50mg/ml lysozyme per gram
μl are fine. 6. The exchange of solution components between the protein sample and the Dialysis Buffer is most efficient if carried out for longer than 1.5 hr. This should be empirically determined. However, an overnight incubation
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