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文献和实验Lymphocyte Transformation 淋巴细胞转染
, each cell line requires several more weeks to reach a cell density of 1 X 108 cells/100 ml.Procedure:Collect 27 ml of anticoagulated blood in 3 yellow top tubes (citrate)， 9 ml each. The blood should be set up in culture as soon as possible for best results
Method: Lymphocyte Transformation
by layering onto histopaque. During centrifugation, erythrocytes and granulocytes are aggregated by ficoll and rapidly settle to the bottom of the tube; lymphocytes and other mononuclear cells remain at the plasma-histopaque interface. Erythrocyte
supernatant through one layer of miracloth into a new 50 ml orange cap tube. Add 1/9 volume of 3.3 M sodium acetate, pH 6.1 (I think you really don't have to do this, but I always do anyway before the isopropanol ppt) and 0.5 X (of the second volume) cold
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